电离辐射通过产物生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简介 ：探讨辐射源确实可通过转化生长因子-β（TGF-β）-介导的上皮-间质转成 (EMT)来倡导细胞的侵扰迁移。使用年均2Gy(60)Coγ本站紫外光源自人类内脏的6种细胞，记录与EMT相关的变化，这包括分别借助显微镜高效率，蛋白质特罗斯季亚涅齐方法，免疫荧光高效率，划痕测试和Transwell于大测试来检视并样品细胞组织形态，EMT标识，侵扰迁移能够等。采用核糖体联免疫吸附法样品这些细胞中都TGF-β蛋白水平，借助特别抑制剂SB431542来指标TGF-β瞬时通路在辐射源EMT中都的效用。经过年均为2Gy紫外光的细胞中都存在间叶细胞的解读，与骗紫外光组相比其上皮标识下降，间叶细胞标识增加，同时其侵扰转移能够增强，TGF-β蛋白水平也提高。进一步发现由A549辐射源诱导的EMT可通过对TGF-β瞬时抑制发生逆转。这些结果表明TGF-β介导的EMT在辐射源诱导增强细胞侵扰转移能够中都起着关键效用。

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